Coding
slipper

Part:BBa_K330001:Design

Designed by: XIE Chensu   Group: iGEM10_HKUST   (2010-10-07)

Protein secretor in Lactobacillus Plantarum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

18bp of original sequence before start codon are chosen as RBS. The original start codon of Signal Peptide lp_0297 is "gtg", which has not been changed to "atg" while making it into our part. The sequence between NdeI and HindIII is randomly chosen as "ttgata".

Source

The sequence of Signal Peptide lp_0297 came from the genome of Lactobicillus Plantarum WCFS1. The gene sequence of Flag-tag is artificially designed according to its peptide sequence.

References

Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1 (Geir Mathiesen*1, Anita Sveen1, May Bente Brurberg2, Lasse Fredriksen1, Lars Axelsson3 and Vincent GH Eijsink1)